Recommended Protocols
A set of Protocols and Applications that are recommended for use with AbD Serotec Antibodies.
- #01 – Direct Immunofluorescence Staining for Flow Cytometry
- #02 – Indirect Immunofluorescence Staining for Flow Cytometry
- #03 – Direct Immunofluorescence Staining of Immunoglobulin Light Chains on B Lymphocytes in Whole Blood by Flow Cytometry
- #04 – Indirect Immunostaining of Frozen Tissue Sections
- #05 – Streptavidin – Biotin Immunostaining of Frozen Tissue Sections
- #06 – PAP/APAAP Immunostaining of Frozen Tissue Sections
- #07 – Indirect Immunostaining of Paraffin Embedded Tissue Sections
- #08 – Streptavidin – Biotin Immunostaining of Paraffin Embedded Tissue Sections
- #09 – PAP/APAAP immunostaining of paraffin embedded tissue sections
- #10 – Antigen Retrieval Techniques for use with Formalin-Fixed Paraffin-Embedded Sections
- #11 – Western Blotting
- #12 – General Procedure for Indirect Sandwich ELISA
- #13 – General Procedure for Indirect Sandwich ELISA using Streptavidin-Biotin Detection
- #14 – General Procedure for Direct ELISA
- #15 – Quantitation of Human Cytokines by ELISA using Serotec Matched Pair Reagents
- #16 – Direct Staining of Intracellular Antigens by Flow Cytometry
- #17 – Direct Staining of Intracellular Antigens by Flow Cytometry – Methanol Modification
- #18 – Direct Staining of Intracellular Cytokines by Flow Cytometry
- #19 – Application notes for Mouse anti-Bromodeoxyuridine
- #20 – Protocol for Intracellular Staining with Leucoperm™
- #24 – Immunoprecipitation Protocol
- #25 – Whole Blood Protocol for Analysis of Intracellular Cytokines
- #26 – Digitonin Permeabilisation of Cells for Flow Cytometry
- #29 – Direct Immunofluorescence Staining for Fluorescence Microscopy
- #30 – Direct Staining of Intracellular Antigens by Flow Cytometry – the paraformaldehyde/saponin method
- #32 – Use of the Serotec dendritic cell exclusion cocktail for identification and phenotyping of peripheral blood dendritic cells
- #33 – Fixative recipes
- #34 – Recommended antigen unmasking procedures for F4/80 staining of paraffin-embedded tissues
- #35 – Thymocyte Il-2/PHA Co-Stimulation Assay
